PhD by Research Programme (Neuroscience and Mental Health)

Research Project Title: Optical Interrogation of Cortical Dynamics during Learning and Sleep
Principal Investigator: Dr Hiroshi Makino, Nanyang Assistant Professor, LKCMedicine

Project Description: 
Learning is an ability to flexibly acquire new skills and knowledge through changes in the structure and function of the nervous system. Although this process is known to be facilitated by sleep, its mechanical underpinnings are still not well understood. Using two-photon calcium imaging, we will simultaneously probe the activity of tens of thousands of neurons across different areas of the mouse cortex during learning and sleep. With this approach, we aim to uncover general principles underlying learning-related transformations of the mesoscopic cortical dynamics. Once such principles are found, we will investigate how sleep can facilitate the process.

Our laboratory integrates various cutting-edge technologies, including imaging with genetically encoded calcium and voltage indicators, optogenetics, pharmacogenetics and virus mediated circuit mapping to investigate the link between neural circuits and behavior. Because of the nature of our approach and a long-standing interest in developing new experimental and analytical tools, we work closely with researchers with diverse backgrounds.

Students will acquire unique expertise in systems neuroscience, which will be useful to further pursue an academic career.

If you have questions regarding this project, please email the Principal Investigator, Dr Hiroshi Makino, Nanyang Assistant Professor, at hmakino@ntu.edu.sg

Please refer to the Lee Kong Chian School of Medicine website for programme information - http://www.lkcmedicine.ntu.edu.sg/Pages/default.aspx