Skip to main content

This job has expired

PhD by Research Programme (Cross-Cutting Research)

Employer
Global Academy Jobs
Location
Singapore
Closing date
Jan 31, 2017

Job Details

Research Project Title: Transcriptome Responses to Bisphosphonate and Infection during Bone Fracture Repair

Principal Investigator: Asst Prof Tom Carney, LKCMedicine

Co-supervisor: Dr Jiang Lei, SGH – Orthopaedics

Project Description:

Bone fractures are common yet the epidemiology is shifting due to increasing population age and prevalence of Type-2 Diabetes, which both reduce bone strength. Furthermore there is increasing rates of fracture complications due to untreatable fracture infections, whilst the diversity of drugs given to elderly or diabetic patients has unknown effects on fracture predisposition or outcomes.

We have developed and characterized a model for fracture modelling in a vertebrate that combines high-throughput and ease of intervention. We have shown that this can model effects of infection, bone disease, and anti-resorptive drugs on fracture incidence and repair. It allows an unprecedented ability to interrogate the molecular changes in cells of the fracture callus under normal and perturbed conditions over time, which would represent the first high detailed transcriptomics analysis of cellular response to fracture.

The aims are:
1. Comparison of cellular response changes and repair rates in fractures with and without staphylococcus infection
2. Comparison of effect of extended  prior exposure to bisphosphonates or PTH on fracture type, repair rates and cell composition
3. Comparative transcriptomics (by RNA-Seq) of infected and uninfected fractures during repair stages
4. Localisation of RNA changes to host cell type or bacteria
5. Comparative transcriptomics of bone exposed to bisphosphonates or PTH  for extended periods
6. Interrogate clinical fracture and osteomyelitis  samples for significant markers identified above
7. Generate genetic mutants of significant upregulated genes using CRISPR/Cas9 to test for function in  bone fracture repair

If you have questions regarding this project, please email the Principal Investigator, Asst Prof Tom Carney, at tcarney@ntu.edu.sg.

Company

Global Academy Jobs works with over 250 universities worldwide to promote academic mobility and international research collaboration. Global problems need international solutions. Our jobs board and emails reach the academics and researchers who can help.

"The globalisation of higher education continues apace, driving in turn the ongoing development of the global knowledge economy, striving for solutions to the world’s problems and educating a next generation of leaders and contributors."

Company info
Website

Get job alerts

Create a job alert and receive personalized job recommendations straight to your inbox.

Create alert