PhD by Research Programme (Cross-Cutting Research)
- Employer
- Global Academy Jobs
- Location
- Singapore
- Closing date
- Jan 31, 2017
View more
- Sector
- Business and Finance, Science, Computer Science and IT
- Hours
- Full Time
- Organization Type
- University and College
- Jobseeker Type
- Academic (e.g. 'Lecturer')
Job Details
Principal Investigator: Asst Prof Tom Carney, LKCMedicine
Co-supervisor: Dr Jiang Lei, SGH – Orthopaedics
Project Description:
Bone fractures are common yet the epidemiology is shifting due to increasing population age and prevalence of Type-2 Diabetes, which both reduce bone strength. Furthermore there is increasing rates of fracture complications due to untreatable fracture infections, whilst the diversity of drugs given to elderly or diabetic patients has unknown effects on fracture predisposition or outcomes.
We have developed and characterized a model for fracture modelling in a vertebrate that combines high-throughput and ease of intervention. We have shown that this can model effects of infection, bone disease, and anti-resorptive drugs on fracture incidence and repair. It allows an unprecedented ability to interrogate the molecular changes in cells of the fracture callus under normal and perturbed conditions over time, which would represent the first high detailed transcriptomics analysis of cellular response to fracture.
The aims are:
1. Comparison of cellular response changes and repair rates in fractures with and without staphylococcus infection
2. Comparison of effect of extended prior exposure to bisphosphonates or PTH on fracture type, repair rates and cell composition
3. Comparative transcriptomics (by RNA-Seq) of infected and uninfected fractures during repair stages
4. Localisation of RNA changes to host cell type or bacteria
5. Comparative transcriptomics of bone exposed to bisphosphonates or PTH for extended periods
6. Interrogate clinical fracture and osteomyelitis samples for significant markers identified above
7. Generate genetic mutants of significant upregulated genes using CRISPR/Cas9 to test for function in bone fracture repair
If you have questions regarding this project, please email the Principal Investigator, Asst Prof Tom Carney, at tcarney@ntu.edu.sg.
Company
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